EDU 25 - Single Cell RNA Sequencing and Its Applications in Radiation Oncology

Moderator(s)

• 

  Aadel Chaudhuri, MD, PhD

  Mayo Clinic Alix School of Medicine
  Rochester, Minnesota, United States
• 

  Lei Xing, PhD, FASTRO

  Stanford University
  Stanford University, CA, United States

Single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics stand at the forefront of precision medicine and hold significant potential to change the landscape of radiobiology and radiation oncology. The technologies allow for molecular characterization at the individual cell level and in the spatial context, providing nuanced insights into the heterogeneity of tumors and therapeutic response, and provide a deeper understanding of various underlying biological processes. The purpose of this session is to provide clinicians in practice and trainees with fundamental and practical knowledge of scRNA-seq for radiobiology and radiation oncology applications. Along this line, the following topics will be discussed: (1) Introduction of scRNA-seq and spatial transcriptomics and single cell biology; (2) Recent advances in bioinformatics of high-dimensional genetic sequencing data analysis; and (3) Identification of biomarkers (such as specific cellular subpopulations with distinct responses to radiation) and other applications of single cell technology. Overall, the development of scRNA-seq and spatial transcriptomics pave the way for personalized treatment strategies, and timely integration of technologies in radiation oncology not only enhances our comprehension of tumor biology but also holds immense promise for tailoring therapeutic interventions to the unique molecular signatures of each patients cancer, ultimately optimizing treatment outcomes.

Presentations:

• 3:00 PM – 3:02 PM ET

  Introductions
  

  Speaker: Aadel Chaudhuri, MD, PhD – Mayo Clinic Alix School of Medicine
• 3:02 PM – 3:18 PM ET

  Introduction to scRNA-seq, Spatial Transcriptomics and Single-Cell Biology
  

  Speaker: Aadel Chaudhuri, MD, PhD – Mayo Clinic Alix School of Medicine
• 3:18 PM – 3:34 PM ET

  Recent Advances in Bioinformatics of High-Dimensional Genomic Sequencing Data Analysis
  

  Speaker: Lei Xing, PhD, FASTRO – Stanford University
• 3:34 PM – 3:50 PM ET

  Single-Cell Technology in Translational Radiation Oncology: Elucidating Molecular Mechanisms of Tumor-Immune Dynamics and Evolution in Response to Radiation in Prostate Cancer
  

  Speaker: Sophia Kamran, MD – Massachusetts General Hospital
• 3:50 PM – 4:00 PM ET

  Questions and Answers
  

  Speaker: Lei Xing, PhD, FASTRO – Stanford University